A describe what you observed in your plaque smear wet mount, direct stained slide, and indirectly stained slide c describe what you observed in your yeast wet. Home / study / science / biology / biology questions and answers / what would be observed in a plaque smear wet mount,direct stained slide and indirectly stained question : what would be observed in a plaque smear wet mount,direct stained slide and indirectly stained sl. Several pathogenic species of fungi that exhibit either yeast (or yeast like) and filamentous form are referred to as being dimorphic the fungi are designated as thermally dimorphic 10 the dimorphism is temperature dependent.
Gdescribe what you observed in your plaque smear wet mount, direct stained slide, and indirectly stained slide idescribe what you observed in your yeast wet. A streak plate involves the progressive dilution of an inoculum of bacteria or yeast over the surface of solidified agar medium in a petri dish the result is that some of the colonies on the plate grow well separated from each other. Microbiology microbiology a list three reasons why you might choose to stain a particular slide rather than view it as a.
Malassezia furfur is a fungus, specifically a yeast, that is approximately 15-45 μm wide and 2-6 μm long it is spherical (coccal) in shape and has a distinguishing bottleneck at one end it is spherical (coccal) in shape and has a distinguishing bottleneck at one end. F describe what you observed in your plaque smear wet-mount, direct stained slide, and indirectly stained slide what were the similarities what were the differences. Microbiology labs 0 list three reasons why you might choose to stain a particular slide rather than view it as a describe what you observed in your plaque smear.
Clinical-mycologic diagnosis of oral yeast infections ingar olsen and aksel stenderup department of microbiology, dental faculty, university of oslo, oslo, norway, and. The pap smear, although specific, is insensitive, with positive results in only about 25 percent of patients with culture-positive symptomatic vulvovaginal candidiasis14 a rapid yeast detection. Mycosis fungoides, also known as alibert-bazin syndrome or granuloma fungoides, is the most common form of cutaneous t-cell lymphoma it generally affects the skin, but may progress internally over time. D explain the similarities and differences between the cells in the plaque and those in the yeast smears e describe the size and shape of the types of cells that you were able to see in the smear. At the initial visit, findings from the potassium hydroxide touch-preparation smear from the right popliteal fossa plaque revealed large 5- to 15-μm-diameter refractile ovoid to circular yeast forms with occasional broad-based budding.
Take a clean cotton swab and gently scrape the inside of your mouth smear the cotton swab on the centre of the microscope slide for 2 to 3 seconds add a drop of methylene blue solution and place a coverslip on top concentrated methylene blue is toxic if ingested wear gloves and do not allow. Difference in plaque smear and yeast smear a wet mount can detect trichomonas, a parasite that causes trichomoniasis, a sexually transmitted disease. The organisms are heat fixed by passing an air-dried smear of the organisms through the flame of a gas burner the heat coagulates the organisms' proteins causing the bacteria to stick to the slide be very careful not to over heat the organisms when fixing them to a slide. Examining the vaginal discharge under the microscope can help distinguish bacterial vaginosis from infections such as yeast vaginitis (vaginal yeast infection, candidiasis) and trichomoniasis a sign of bacterial vaginosis under the microscope is a vaginal cell called a clue cell.
Cytologic smear samples should be prepared chairside, and then submitted to a laboratory for microscopic diagnosis tissue biopsy should be performed to rule out other epithelial conditions, if not responsive to antifungal therapy. Chronic plaque-like oral candidiasis (chronic hyperplastic oral candidiasis): persistent firm, white plaques on the cheek or tongue, that are not easily removed it is most common in smokers and men aged 30 years. Yeast, a type of fungi (plural for fungus), is found in many places from nature, to research labs and even everyday kitchens for baking yeast colonies generally look similar to bacterial colonies some species, such as candida , can grow as white patches with a glossy surface.
For a wet mount, scrapings or smears obtained from skin, nails, or oral or vaginal mucosa are examined under the microscope for hyphae, pseudohyphae, or budding yeast cells a potassium hydroxide smear, gram stain, or methylene blue is useful for direct demonstration of fungal cells. Detection of streptococcus mutans in plaque samples by the direct fluorescent antibody test walter j loesche and ella grenier dental research institute and department of oral biology, university of. Yeast infections, and in particular, intestinal candida yeast infections (and systemic candidiasis), can lurk for years with few overt symptoms that allow a diagnosis consequently, medical diagnostic lab tests that irrefutably identify a yeast infection are vital in order for your doctor to be convinced, and for you to receive proper treatment.